|Alternate:||AFB Culture and Smear; Mycobacterium Culture; Atypical Mycobacterium Culture and Smear|
|Includes:||Culture and acid fast stain Susceptibility by special request|
|Available:||Daily, 24 hours|
|Turnaround:||Preliminary report at 1 week. Negative cultures are finalized at 6 weeks, positives as identified.|
|Specimen:||Sputum, bronchial washing, urine, gastric aspirate, wound, abscess or surgical tissue|
|Volume:||Sputum-- 5.0 mL first morning specimen|
Urine --50 mL first morning void
Gastric aspirate, fasting sample--5.0ml
Wound or abscess----culturette or aspirate Surgical tissue---sterile container
|Container:||Culturette or sterile container|
|Temperature:||Room temperature transport to lab within 30 minutes|
|Rejection:||Saliva instead of sputum
Sputum collected on swab
|Normal:||No acid fast organisms seen on smear No acid fast organisms isolated at 6 weeks|
|Uses:||Identify microorganisms that may cause infection.|
|Limitations:||Antibiotic therapy may inhibit growth. |
Culture is more sensitive than stain.
Contamination with adjacent areas should be avoided.
Notify lab if M. xenopi or M.marinum suspected
Laboratory recommends collecting 3 sputum specimens for acid-fast smears and cultures in patients with clinical and chest X-Ray findings compatible with tuberculosis. These 3 samples should be collected at 8 - 24 hour intervals (24 hours when possible) and should include at least one first morning specimen.
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