Lab Test : Acid Fast Culture and Smear

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Alternate:AFB Culture and Smear; Mycobacterium Culture; Atypical Mycobacterium Culture and Smear
Includes:Culture and acid fast stain Susceptibility by special request
Phone:(937) 641-3902
Available:Daily, 24 hours
Turnaround:Preliminary report at 1 week. Negative cultures are finalized at 6 weeks, positives as identified.
Specimen:Sputum, bronchial washing, urine, gastric aspirate, wound, abscess or surgical tissue
Volume:Sputum-- 5.0 mL first morning specimen
Urine --50 mL first morning void
Gastric aspirate, fasting sample--5.0ml
Wound or abscess----culturette or aspirate Surgical tissue---sterile container
Container:Culturette or sterile container
Temperature:Room temperature transport to lab within 30 minutes
Rejection:Saliva instead of sputum
Sputum collected on swab
Normal:No acid fast organisms seen on smear No acid fast organisms isolated at 6 weeks
Uses:Identify microorganisms that may cause infection.
Limitations:Antibiotic therapy may inhibit growth.
Culture is more sensitive than stain.
Contamination with adjacent areas should be avoided.
Notify lab if M. xenopi or M.marinum suspected

Laboratory recommends collecting 3 sputum specimens for acid-fast smears and cultures in patients with clinical and chest X-Ray findings compatible with tuberculosis. These 3 samples should be collected at 8 - 24 hour intervals (24 hours when possible) and should include at least one first morning specimen.

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