Blood Test: Hemoglobin Electrophoresis

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What It Is

A hemoglobin electrophoresis test measures the different types of hemoglobin in the bloodstream. Hemoglobin, the oxygen-carrying protein inside red blood cells, comes in many molecular forms, some normal and some abnormal. Normal hemoglobin carries and releases oxygen efficiently, while abnormal hemoglobin doesn't.

The most common types of normal hemoglobin are:

  • Hemoglobin F, the normal type found in fetuses and newborns. It's replaced by hemoglobin A soon after birth.
  • Hemoglobin A, the normal type most commonly found in healthy kids and adults.

The most common types of abnormal hemoglobin are:

  • Hemoglobin S, C, D, E, M

If a person inherits the genes that cause production of too much of an abnormal type of hemoglobin, or not enough normal hemoglobin, it can lead to blood disorders, including:

  • Sickle cell disease. Hemoglobin S is responsible for most types of sickle cell disease, a condition in which the red blood cells have a crescent or sickle shape that causes them to break down too quickly and clog small blood vessels.
  • Thalassemias. This group of genetic blood diseases affects the amount and type of hemoglobin made (for example, too much hemoglobin F and not enough hemoglobin A in a child).

The electrophoresis process takes advantage of the fact that hemoglobin types have different electrical charges. During electrophoresis, an electrical current is passed through the hemoglobin in a blood sample, which causes the hemoglobin types to separate at different rates and form bands. By comparing the pattern formed with that of a normal blood sample, doctors can see the types and quantities of hemoglobin present in the blood sample.

Why It's Done

A doctor may order hemoglobin electrophoresis to help diagnose diseases (called hemoglobinopathies) involving abnormal hemoglobin production, such as sickle cell disease and thalassemia.

Doctors also may order the test when a child has family history of a hemoglobinopathy or is found to have anemia that isn't due to a more common cause, such as iron deficiency.

In many states, a hemoglobin electrophoresis is performed as part of a series of newborn screening blood tests, primarily so that kids with sickle cell anemia can be diagnosed, monitored, and treated early to prevent potentially life-threatening complications.


No special preparation is necessary. However, if your child has had a transfusion in the last 3 months, hemoglobin levels may be altered, so let the doctor know.

On the day of the test, having your child wear a T-shirt or short-sleeved shirt can make things easier for your child and the technician who will be drawing the blood

The Procedure

A health professional usually draws the blood from a vein. For an infant, the blood may be obtained by puncturing the heel with a small needle (lancet).

If the blood is being drawn from a vein, the skin is cleaned with antiseptic and an elastic band (tourniquet) is placed around the upper arm to apply pressure and cause the veins to swell with blood. A needle is inserted into a vein (usually in the arm inside of the elbow or on the back of the hand) and blood is withdrawn and collected in a vial or syringe.

After the procedure, the elastic band is removed, then the needle is removed and the area is covered with cotton or a bandage to stop the bleeding. Collecting the blood for the test will only take a few minutes.



What to Expect

Either method (heel or vein withdrawal) of collecting a blood sample is only temporarily uncomfortable and can feel like a quick pinprick. Afterward, there may be some mild bruising, which should go away in a day or so.

Getting the Results

The blood sample will be processed by a machine. The results are usually available after 1-2 days.


Hemoglobin electrophoresis is considered a safe procedure. However, some problems can occur with having blood drawn, including:

  • fainting or feeling lightheaded
  • hematoma (blood accumulating under the skin causing a lump or bruise)
  • pain associated with multiple punctures to locate a vein

Helping Your Child

Having a blood test is relatively painless. Still, many kids are afraid of needles. Explaining the test in terms your child can understand might help ease some of the fear.

Allow your child to ask the technician any questions he or she might have. Tell your child to try to relax and stay still during the procedure, as tensing muscles and moving can make it harder and more painful to draw blood. It also may help for your child to look away when the needle is being inserted into the skin.

If You Have Questions

If you have questions about the hemoglobin electrophoresis test, speak with your doctor. You can also talk to the technician before the procedure.

Reviewed by: Steven Dowshen, MD
Date reviewed: July 2014

Related Resources

OrganizationNational Heart, Lung, and Blood Institute (NHLBI) The NHLBI provides the public with educational resources relating to the treatment of heart, blood vessel, lung, and blood diseases as well as sleep disorders.
OrganizationSickle Cell Disease Association of America This group provides education, advocacy, and other initiatives to promote awareness of and support for sickle cell disease programs.
OtherSickle Cell Information Center The mission of this site is to provide patient and professional education, news, research updates, and sickle cell resources.
OrganizationCooley's Anemia Foundation This organization provides information about Cooley's Anemia and other forms of the genetic blood disorder thalassemia.
OrganizationAmerican Academy of Pediatrics (AAP) The AAP is committed to the health and well-being of infants, adolescents, and young adults. The website offers news articles and tips on health for families.
Web SiteLab Tests Online This non-commercial site was developed by laboratory professionals to educate caregivers, patients, and patients' families about lab tests.

Related Articles

Alpha Thalassemia Alpha thalassemia is a blood disorder in which the body has a problem producing alpha globin, a component of hemoglobin, the protein in red blood cells that transports oxygen throughout the body.
Beta Thalassemia Beta thalassemia is a blood disorder in which the body has a problem producing beta globin, a component of hemoglobin, the protein in red blood cells that transports oxygen throughout the body.
Anemia Anemia, one of the more common blood disorders, occurs when the number of healthy red blood cells decreases. This can result in a variety of symptoms, including fatigue and stress on all the body's organs.
Newborn Screening Tests Newborn screening tests look for harmful or potentially fatal disorders that aren't apparent at birth. Find out which tests are done and which disorders they're designed to detect.
Blood Transfusions A blood transfusion is a safe and relatively simple medical procedure that replaces blood lost during surgery or because of an injury or illness.
Sickle Cell Disease Thanks to advancements in early diagnosis and treatment, most kids born with sickle cell disease grow up to live relatively healthy and productive lives.
Blood Test: Hemoglobin Hemoglobin is a protein in red blood cells that transports oxygen throughout the body. A hemoglobin test may be performed as part of a routine health exam, during a time of illness, or to detect anemia.

Note: All information is for educational purposes only. For specific medical advice, diagnoses, and treatment, consult your doctor.

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