Lab Test : Transfusion Reaction Investigation

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Alternate:Hemolytic Reaction Workup
Includes:DAT and examination of: pre- and post- reaction serum for hemolysis and a check of all clerical work for possible error. Evaluation by pathologist.

If the above tests are negative, no additional tests are required. If the results of the initial investigation are positive, then the following may be indicated: gram stain and culture of donor unit: repeat blood type, DAT, antibody screen and crossmatches with pre- and post- reaction specimens; antibody ID; BUN, creatinine; bilirubin or haptoglobin.
Department:Transfusion Services
Phone:(937) 641-3369
SQ Code:(TFRX): Do not order in SunQuest. Call first
Available:Daily, 24 hours
Turnaround:30 - 60 minutes
Specimen:Blood, urine, donor unit (if indicated by Transfusion Service)
Volume:2.5 mL blood
1 mL urine
Container:Purple top tube or 3 purple Microtainers, urine container, and donor unit
Temperature:Room temperature for transport. Refrigerated for storage.
Rejection:Improper labeling or wrong collection tube.
Normal:No evidence of red cell incompatibility.
Uses:Investigate cause of possible transfusion reactions to blood products. See Transfusion Reaction Table.
Limitations:Reactions to white cells, platelets, or plasma proteins are not detected in the standard workup. Non-immunohematologic sources, such as in-vitro hemolysis of donor cells, may also cause hemolysis suggesting a false transfusion reaction.
Method:Hemagglutination
 

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