|Alternate:||Hemolytic Reaction Workup|
|Includes:||DAT and examination of: pre- and post- reaction serum for hemolysis and a check of all clerical work for possible error. Evaluation by pathologist.|
If the above tests are negative, no additional tests are required. If the results of the initial investigation are positive, then the following may be indicated: gram stain and culture of donor unit: repeat blood type, DAT, antibody screen and crossmatches with pre- and post- reaction specimens; antibody ID; BUN, creatinine; bilirubin or haptoglobin.
|SQ Code:||(TFRX): Do not order in SunQuest. Call first|
|Available:||Daily, 24 hours|
|Turnaround:||30 - 60 minutes|
|Specimen:||Blood, urine, donor unit (if indicated by Transfusion Service)|
|Volume:||2.5 mL blood |
1 mL urine
|Container:||Purple top tube or 3 purple Microtainers, urine container, and donor unit|
|Temperature:||Room temperature for transport. Refrigerated for storage.|
|Rejection:||Improper labeling or wrong collection tube.|
|Normal:||No evidence of red cell incompatibility.|
|Uses:||Investigate cause of possible transfusion reactions to blood products. See Transfusion Reaction Table.|
|Limitations:||Reactions to white cells, platelets, or plasma proteins are not detected in the standard workup. Non-immunohematologic sources, such as in-vitro hemolysis of donor cells, may also cause hemolysis suggesting a false transfusion reaction.|
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