|Includes:||Microscopic examination of Wright-Giemsa-stained specimen|
|Department:||Pathology, Histopathology Laboratory|
|Available:||Mon - Fri, first sift|
|Temperature:||Room temperature, air dried|
|Rejection:||Improper handling, e.g., spray fixing with alcohol.|
|Uses:||To rule out Herpes, multinucleated giant cells.|
|Limitations:||Improperly prepared slides, using a vesicle that is crusted, or not scraping the base of the lesion. Acantholytic cells, which are rounded epidermal cells with large nuclei and scant cytoplasm may be seen on a Tzanck smear. This finding suggests pemphigus vulgaris, an autoimmune blistering disorder. A confirmatory biopsy is necessary when a finding of acantholyic cells is made . |
Label the slides with the patient's name.
Select the lesion site.
Using gloves, cleanse the site with gauze or swab soaked in water.
Incise the top of a vesicle with a sterile scalpel and lightly apply the
specimen to the middle of a glass slide.
Lightly scrape the base of the vesicle and apply the lesion material to the
middle of another glass slide. Smear an area about the size of a dime, i.e.,
just enough to be covered by a No. 1 (22 x 22 mm) cover slip.
Prepare two or more smears if necessary.
|Method:||Microscopic examination. See "Limitations" below for preparation of sample.|
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