Lab Test : DAT

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Alternate:Direct Coombs; Direct Antiglobulin Test
Includes:Direct Antiglobulin Test with polyspecific (anti-IgG and anti-C3d) antiglobulin serum. If the polyspecific test is positive, a DAT is performed with anti-IgG antiglobulin serum. Tests for anti-C3d can be sent to a reference lab upon request.
Department:Transfusion Services
Phone:(937) 641-3369
SQ Code:DAT
Available:Daily, 24 hours
Turnaround:1 hour
Specimen:Blood
Volume:0.5 mL
Container:Purple top tube or 1 purple Microtainer
Temperature:Room temperature for transport. Refrigerated for storage.
Rejection:Improper labeling or wrong collection tube.
Normal:Negative
Uses:Diagnosis of Hemolytic Disease of the Newborn, Delayed Transfusion Reaction investigation, and Autoimmune Hemolytic Anemia.
Limitations:In rare cases of Autoimmune Hemolytic Anemia, hemolysis may occur with very low levels of red cell-bound autoantibody that is not detectable with routine methods. The presence of potent cold autoagglutinins may interfere with test interpretation. Testing performed using clotted blood specimens may yield false-positive results with anticomplement.
Method:Hemagglutination
 

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