|Alternate:||Direct Coombs; Direct Antiglobulin Test|
|Includes:||Direct Antiglobulin Test with polyspecific (anti-IgG and anti-C3d) antiglobulin serum. If the polyspecific test is positive, a DAT is performed with anti-IgG antiglobulin serum. Tests for anti-C3d can be sent to a reference lab upon request.|
|Available:||Daily, 24 hours|
|Container:||Purple top tube or 1 purple Microtainer|
|Temperature:||Room temperature for transport. Refrigerated for storage.|
|Rejection:||Improper labeling or wrong collection tube.|
|Uses:||Diagnosis of Hemolytic Disease of the Newborn, Delayed Transfusion Reaction investigation, and Autoimmune Hemolytic Anemia.|
|Limitations:||In rare cases of Autoimmune Hemolytic Anemia, hemolysis may occur with very low levels of red cell-bound autoantibody that is not detectable with routine methods. The presence of potent cold autoagglutinins may interfere with test interpretation. Testing performed using clotted blood specimens may yield false-positive results with anticomplement.|
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