Lab Test : Clostridium Difficile by PCR

Print this page Bookmark and Share
Alternate:C diff by PCR
Includes:PCR testing for toxigenic C. Difficile
Department:Virology
Phone:(937)641-3907
SQ Code:PCRCD
Available:Orderable daily, 24 hours. Specimens received before 1 PM resulted the same day or next day.
Turnaround:1 -2 days.
Specimen:Stool
Volume:1 mg of stool (equivalent to the size of a pea), 1 mL liquid stool, or a swab heavily coated with stool.
Container:Clean container or culturette
Temperature:Room temperature. Refrigerate if transport is delayed more than one hour.
Rejection:Formed stool should not be tested.
Normal:Negative for C. difficile by PCR.
Uses:Detects the presence of cytotoxin producing C. difficile.
Limitations:The C. difficile PCR assay is a highly sensitive test that detects the Toxin B gene. This test has been approved by the Food and drug Administration for testing unformed stool specimens from patients suspected of having C. difficile infection and has been validated by the Childrens Medical Center Virology Laboratory. this test detects the presence of DNA encoding this gene and does not detect viable organisms or the presence of the protien toxin. As a result, PCR is not to be used as a test of cure and repeat testing on POSITIVE patients will only be accepted for new episodes after 7 days from the previous test.

Also due to the sensitivity of the PCR test the negative predictive value in our population is very good. Repeat testing to confirm NEGATIVE results is not necessary and will only be accepted for new episodes of diarrhea that occur after 7 days from the previous test.

In certain age groups the sole determination of C. difficile toxin positivity in stool is not conclusive evidence that the patient has C. difficile infection (CDI). Asymptomatic carriage of toxigenic C. difficile is common in young children (<2 years of age) and laboratory findings should be interpreted in conjunction with patient history to establish a diagnosis of CDI.
Method:Polymerase Chain Reaction (PCR)
 

Sign up for Pediatric Link

Finding the latest trusted child health and safety information doesn't have to be hard. Pediatric Link, Dayton Children's e-newsletter offers timely and up-to-date information for health care professionals from the experts you trust at Dayton Children's.  Sign up

Upcoming Events

Fun family health fair

REGISTRATION: Sign up online at www.keysports.net. By mail: Fill out this entry form and mail with a check payable to Miamisburg High School to Miamisburg High School C/O Jenny Brockert 1860 Belvo Road Miamisburg, OH 45342 For more information all Jenny Brockert at (937) 866-0771 ext.1414 or email her at jbrockert@miamisburg.k12.oh.us

Dayton Children’s cordially invites you to attend our upcoming state-wide pediatric trauma symposium. The symposium will be held at the Wyndham Hotel, Miamisburg, OH on April 25, 2014.

View full event calendarView full event calendar

The Right Experts

Just right for all kids

Accreditations

The Children's Medical Center of Dayton Dayton Children's
The Right Care for the Right Reasons

One Children's Plaza - Dayton, Ohio - 45404-1815
937-641-3000
www.childrensdayton.org