Lab Test : Bilirubin, Total and Direct

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Alternate:Bilirubin, Conjugated and Unconjugated
Includes:Measured total and direct bilirubin, and calculated indirect bilirubin.
Department:Chemistry
Phone:(937) 641-3428
SQ Code:BILI DBIL-direct TBIL-total
Available:Daily, 24 hours
Turnaround:Routine: 3 hours, STAT: 30 minutes
Specimen:Blood
Volume:0.5 mL
Container:3 mL green top tube or green microtainer (sodium heparin)
Temperature:Transport to lab within 30 minutes, protect from light.
Rejection:None
Normal:Tbil:
0 days: <8.0 mg/dL
2 days:<12.0 mg/dL
3 days: <16.0 mg/dL< br>1 month: 0.2 - 1.0 mg/dL.
Dbil:
1 month 0-0.4mg/dL
Uses:Liver and biliary test useful in the differential diagnosis of jaundice from bilirubin overproduction (hemolysis), decreased uptake (Gilbert's), decreased conjugation (hepatocellular disease, neonatal immaturity, Crigler-Najjar, drug inhibition), decreased excretion into bile (hepatocellular disease, familial, drug induced, pregnancy, obstructive bile duct disease).
Limitations:Visibly hemolyzed specimens will cause interference in the direct bilirubin result.
Method:Colorimetric biochromatic endpoint with blank (Jendrassic and Grof method)
 

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